Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Thermo Scientific Production Chemicals and Services 50 MM TRIS-HCL 10 MM EDTAPH8
NC3588273 50 MM TRIS-HCL 10 MM EDTAPH8
Biotang Inc TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml
TRIS-HCL Buffer 1.0M, pH 8.2 (0.2 micron filtered), 500 ml.
Cayman Chemical Tris(4,7-diphenyl-1,10-phenanthroline)ruthenium II dichloride complex, Purity: ≥95%, CAS: 36309-88-3, 25 mg
A luminescent probe (absorption λmax: 455 nm, luminescence λmax: 613 nm) widely used for detection and quantitation of oxygen; strongly reduced by molecular oxygen as a result of dynamic quenching; thus oxygen detection can be based on either measurement of intensity or decay time
Biotang Inc HEPES Lysis Buffer with Triton (2X), 125 ml
HEPES Lysis Buffer with Triton (2X), 125 ml.
TARGETMOL CHEMICALS INC P62-mediated mitophagy inducer
Also available in 1 mL, 2 mg, 5 mg, 10 mg, 50 mg, 100 mg, 200 mg and bulk. Please contact Fisher for quotes. P62-mediated mitophagy inducer (PMI) is a regulator of mitophagy. Purity 99.35%
Teknova 1M Tris-HCl, pH 7.9. 1000mL, Sterile. 2 pack.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Complement Technology Inc GVBE (GVBo with EDTA) 250ml
GVBE is an isotonic buffer used in complement assays to prevent complement activation or to stop ongoing complement activation when added at the end of an incubation
Biotang Inc BioAcryl-P (30%) Bis-Acrylamide 29:1, 1000 ml
BioAcryl-P (30%) Bis-Acrylamide 29:1, 1000 ml.
Bioworld TT - SDS Running Buffer (Anode) (10X), 1 L
Product DescriptionTris-Tricine (TT)-SDS running buffer has a 10X concentration and a pH of 8.9. It contains 1M Tris-base, 1M Tricine and 1% SDS.The tricine system requires SDS in sample and running buffers for best results. Tricine-SDS PAGE is commonly used to separate proteins that range in molecular weight between 1 – 100 kDa but is preferred for the resolution of proteins smaller than 30 kDa.
Bioworld ACE TBE w/ 7M Urea, pH 8.6, 100 mL
Product DescriptionA.C.E. TM TBE (Tris-Borate-EDTA) buffer comes with 7M of Urea with a pH level of 8.6. A.C.E. TM buffers deliver consistent and reproducible results because they are manufactured with the highest quality reagents in nuclease free water. Product is shipped at room temperature.
eMolecules 5704-04-1 | Tricine | Oakwood Chemical | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
Tricine | Oakwood Chemical | 5704-04-1 | MFCD00004277 | 179.172 | C6H13NO5 | 98.000 | OCC(CO)(CO)NCC(O)=O | 1g | 537694332
VWR INTERNATIONAL LLC TRIS-Borate-EDTA Buffer, 10X LIQUID CONCENTRATE, Ultra Pure Grade4l
TRIS-Borate-EDTA Buffer, 10X LIQUID CONCENTRATE, Ultra Pure Grade | 4l
Alkali Scientific CellPro™ TE 1X, pH 8.0, 500 mL
Introducing CellPro™ TE 1X (Tris EDTA), meticulously formulated by Alkali Scientific™ to meet the exacting standards of molecular biology research. This Molecular Biology Grade TE buffer ensures exceptional purity and performance, making it an indispensable tool for various molecular biology applications, including DNA and RNA purification, DNA sequencing, and PCR. With its precisely balanced composition and stringent quality control measures, CellPro™ TE 1X provides researchers with reliable and reproducible results, facilitating accurate and efficient laboratory workflows. Trust CellPro™ TE 1X for your molecular biology experiments and experience the assurance of superior quality and performance in every assay.
Alkali Scientific TRIS CAPS Buffer 10X Solution, 2 x 500 Milliliter Bottles
TRIS CAPS Buffer 10X Solution is designed for use in capillary electrophoresis and other advanced electrophoretic techniques. This formulation, available in two 500mL bottles, ensures convenient preparation and consistent results during capillary separation of charged biomolecules. The 10X solution can be diluted to create the working concentration needed for various electrophoresis applications. TRIS CAPS buffer is particularly useful in separating proteins and nucleic acids in a high-resolution format. This buffer is often used in protein and DNA/RNA electrophoresis protocols, providing stable pH conditions that enhance separation and improve sensitivity during detection.