Life Science Buffers

Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.

Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.

Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.

The choice of buffer for a particular biological reaction or site depends on several factors:

Temperature
Desired or target pH
Buffer toxicity (to the system)
Buffer interactions with other system components

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2,686 – 2,700 of 3,502 results

2,686

Apexbio Technology LLC Protease Inhibitor Cocktail (EDTA-Free 100X in DMSO) 100x1ml

Cellular crude extracts contain numerous endogenous enzymes such as phosphatases and proteases capable of degrading proteins in the extracts The most effective strategy to enhance the yield of intact proteins is through the addition of inhibitors targeting these known endogenous enzymes The Protease Inhibitor Cocktail is intended for the purification of His-tagged proteins to improve protein stability This cocktail functions by inhibiting proteases that degrade either phosphorylated or non-phosphorylated protein substrates This Protease Inhibitor Cocktail contains individual components including AEBSF Bestatin E-64 Phosphoramidon and Pepstatin A offering broad-spectrum specific inhibition against cysteine serine aspartic thermolysin-like proteases and aminopeptidases It has been optimized and validated specifically for use with His-tagged proteins The cocktail is dissolved in DMSO and supplied as a ready-to-use solution

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2,687

Apexbio Technology LLC TUNEL Apoptosis Detection Kit (DAB) 20rxns

The TUNEL Apoptosis Detection Kit (DAB) is designed to detect nuclear DNA fragmentation occurring during apoptosis in tissues or cells During apoptosis endonucleases within cells become activated and cleave genomic DNA between nucleosomes resulting in DNA fragments of approximately 180-200 bp or multiples thereof The fragmented DNA generates free 3 -OH termini at which terminal deoxynucleotidyl transferase (TdT) incorporates biotin-labeled dUTP Subsequently these labeled termini bind to horseradish peroxidase (HRP)-conjugated streptavidin (Streptavidin-HRP) followed by chromogenic visualization using DAB This kit allows direct observation using an ordinary optical microscope and is convenient for use It is applicable to a wide range of sample types including frozen sections paraffin-embedded sections adherent cell cultures and suspended cells making it versatile for apoptosis detection

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2,688

Medchemexpress LLC NaCl, KCl, Phosphate | AR/GR grade | 10 POUCHES

MCE PBS Powder (2 L of 1x) consists of NaCl, KCl, and Phosphate, and is widely used in immunoassay, microbiology, and protein experiments. The PBS buffer prepared from this product can be directly used for cell culture and other related experiments after being sterilized by 0.22 μm filtration. Each pouch can be diluted to 2 L of 1x PBS buffer, resulting in a concentration of 137 mM NaCl, 2.7 mM KCl, and 10 mM Phosphate. This product offers a convenient method for making PBS buffer, eliminating the need to weigh and mix individual components.